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Date: 2/12/97 3:38 PM
To: cyto-inbox
From: Maryalice Stetler-Stevenson
What is the exact concentration of NaOH?
> Reply to: RE>>A clean machine -Reply
>We've been using a dilute sodium hydroxide solution to clean the tubing in
>our three
>FACscans for several years now. We used to use bleach, as recommended by the
>manufacturer, and sometimes we would have clogs which never seemed to clear up
>completely. Our protein chemist recommended NaOH to me late one afternoon when
>I was particularly frustrated with a stubborn clog. The NaOH cleared it right
>out, and from then on we've used it regularly to keep the lines clear with few
>problems. I've always wondered if anyone else has tried it and how it has
>worked for them.
>Florence
>
>--------------------------------------
>Date: 2/11/97 4:21 PM
>To: cyto-inbox
>From: Jim Zanghi
>
>>Small beads staying in the instrument can be a problem. Triton X-100 at
>>0.1% (the same concentration used to permeabilize cells) in water will
>>remove most of them.
>>
>>Betsy Robertson
>>>
>>> Running ethanol
>>> after the beads seems to help push them through more quickly.
>>>
>>> Tony Bakke
>
>I was wondering about what types of solutions can be run through a flow
>cytometer without causing damage or corrosion. Is pure ethanol okay? On
>the advise of others, I have run 50% hot bleach to clear up clogs, but I
>was very weary about doing this since bleach corrodes stainless steel.
>I've always followed with a 5 minute water rinse, but this concentration of
>bleach seems a bit extreme (we routinely use 10% bleach at room temp).
>If 0.1% triton or 70% ethanol is as effective as 50% hot bleach, than this
>seems to be the way to go. Any thoughts about this? What do
>manufacturers recommend?
>
>Jim
>
>--
>James A. Zanghi
>Dept. of Chemical Engineering
>Northwestern University
>Evanston, IL
>
>
>
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>Date: Tue, 11 Feb 1997 10:19:08 -0600
>To: cyto-inbox
>From: zanghi@merle.acns.nwu.edu (Jim Zanghi)
>Subject: Re: A clean machine -Reply
Maryalice Stetler-Stevenson
Director Flow Cytometry Unit
Laboratory of Pathology, NCI, NIH
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Date: Wed, 12 Feb 1997 18:09:45 -0400
To: cyto-inbox
From: stetler@box-s.nih.gov (Maryalice Stetler-Stevenson)
Subject: Re: A clean machine -Reply
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web