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Regarding TSO installation : are you sure that large FSC obscuration bar is
installed. Sometimes it's lack in the kit..
One another question: do you try different drop drive frequencies. When you
change DD frequency you can see differencies in FSC and SSC signals (On my
TSO best signals are obtained with a drop dive at 56600 for 30 PSI without
large bars. Before I've worked with 53100 DD Freq with large obscuration
bars but FSC signal was lower).
Sincerely
DUPERRAY Christophe
INSERM U291
99, rue Puech Villa
34197 MONTPELLIER cedex 5
Tel 33 (0)4 67 63 61 29
Fax 33 (0)4 67 04 18 63
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web