 |
 |
 |
 |
 |
I response to the question of Jan Keij as to how to block a
glutaraldehyde fixation. What about using Tris ? It has free amines
which react avidly with all aldehydes. Just replace your phosphate
buffer with a Tris buffer and your glutaraldehyde will become
"neutralized" in a single wash step.
Molecular Probes, Inc. does not offer Tris for sale nor has any
commercial interests in Tris (in case you would wonder).
Martin Poot
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web