RE: PI staining and washing

TIM KUTE (tkute@bgsm.edu)
Wed, 9 Jul 1997 17:12:06 -0400 (EDT)

To all:
Just as a comment about PI staining. It is not necessary to wash
out the excess stain of PI. It is known that PI is not very fluorescent
unless it is bound to DNA. Therefore, the unbound PI will not be measured
by flow cytometry.

Dr. Tim Kute email: tkute@bgsm.edu
Associate Professor in Pathology Tel#: 910-716-2950
Wake Forest Univ-Bowman Gray School of Medicine Fax#:910-716-7595
Medical Center Blvd
Winston-Salem, NC 27157-1072


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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu