 |
 |
 |
 |
 |
>>> Surgical Labs Resident <SURGLAB1@is.rvh.mcgill.ca>
01/15/97 11:36am >>>
Dear Flow-ers,
Does anyone know of a good 3rd colour marker for detection
of calcium flux on lymphocytes, without having any leakage into
Fl1 and Fl2? Right now we are using a marker called fluo3 and it
really bleeds into the other colours. We are using a single laser
FACScalibur.
Also, for people sorting on the FACScalibur, is there any way
to increase my cell recovory? Right now it is at 10%. Is it more
efficient to use a cell culture insert than a filter memrane( where
I think some of the cell loss is occuring).
Thanks in advance.
Raffi Manoukian
Royal Victoria Hospital
Montreal,Quebec, Canada
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web