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Yes, PE-Cy5 will work fine with FITC and PE as will PerCP. They all have
similar emission maxima at about 670nm, but PerCp may have a slight
advantage as is it not a tandem dye. There are several companies that make
PE-Cyanine conjugates, all giving them different names eg Cychrome
(Pharmingen), TriColor (Caltag), Quantum Red (Sigma) or just PE-Cy5
(Coulter, DAKO). PerCP comes from Becton Dickinson.
As to the range of antibodies available as direct conjugates, Pharmingen,
Caltag and Sigma do a range of anti-human and anti-mouse antibodies while
Coulter and DAKO and BD only do anti-human antibodies (Although I am
prepared to be corrected on this!). Most manufacturers will also do a
streptavidin-conjugated fluorochrome. Your local reps will surely be able
to tell you more!
Hope this is of some help,
Derek
Derek Davies
Imperial Cancer Research Fund, London, UK
http://www.icnet.uk/axp/facs/davies/index.html
On Mon, 2 Dec 1996, Kim Staquet wrote:
>
> Hello,
>
> Continuing on the line of multi-color staining combinations for use with a single laser....
> We want to do triple staining and we have a BD FACScan with an argon laser. Does
> anyone know will PE-cy5 work in combination with FITC and PE? How about PER-CP? If
> so, where can we buy PE-cy5 or PER-CP conjugated goat anti-human or anti-mouse Ig's.
>
> Thanks for your help,
> Kim Staquet
>
> e-mail at Staquetk@Centocor.com
>
>
>
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web