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Jan,
We're doing this for one of our users. Cy3 can be measured using the FL2
detector. It wouldn't be my first choice (or my 2nd or 3rd or...) of a
conjugate to excite at 488 but it seems to work. Perhaps that's just
because their antigen is highly expressed, but I have no data with another
fluorochrome to compare.
This web page has spectra for CY3:
http://optics.jct.ac.il/~aryeh/Spectra/GIFs/Cy3.18.GIF
Good luck,
Neal Benson
-- Neal Benson University of Florida E-mail: neal-benson@ufl.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web