 |
 |
 |
 |
 |
> We have someone who wants to use a monoclonal antibody conjugated to Cy3.
> They have been told you can't use a FACScan as Cy3 won't excite at 488nm.
> Any suggestions which instruments can be used.
You can indeed use Cy3 on a FACScan. We have looked at a dozen or
more in-house conjugates. While Cy3 would *prefer* to be excited at
around 550nm, or even at 515nm, you can still get enough signal using
488nm if the antibody is suitable. Expect up to one decade less signal
than for a comparable PE conjugate.
\ / < The Flow Systems Laboratory
Frank Battye, \__/ <<<<< The Walter & Eliza Hall Institute
Dora Kaminaris,----------!!<<<<<<<< Victoria 3050, AUSTRALIA
Viki Lapatis and /!!\ <<<<< ph: 61_3_9345 2540 fax: 61_3_9347 0852
Jennie Parker o !! \ < in:: "facs_copy@wehi.edu.au"
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web