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you can try abe schwartz's simply cellular beads. call Flow cytometry
standards corp. 800 227-8143. they're fairly easy to use and you can
extrapolate the number of Ab bound to a cell (average of a population
actually)
hope this helps
gary woo
>----------
>From: Ras Smit
>Sent: Wednesday, July 9, 1997 8:59 AM
>To: Cytometry Mailing List
>Subject: Semi-quantitation of CD4 receptors
>
>
>Hallo everybody
>
>I believe a similar question has been recently asked, but can anybody
>tell me how to determine the density of CD4 receptors on lymphocytes
>with flow cytometry. Any previous methods discussed on
>this mailing list or references will be greatly appreciated.
>
>Ras Smit
>Dr EJ Smit; Registrar in Virology, Medical University of Southern Africa
>PO Box 173, MEDUNSA, 0204, South Africa
>Phone +27 12 521-4113 Fax +27 12 521-5794
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
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If you have any comments please direct them to
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Phone: (765)-494-0757;
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