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The "aspiration of unknown volume" problem IS preventable!
The sample uptake assembly in these instruments is actually two
concentric tubes; the inner carries the cell suspension into the
instrument, the outer is a drip catcher to suck away the backflush.
Simply remove the outer layer of the uptake tube and the sheath
fluid backflush is no longer sucked away, neither is the sample.
Of course there are many implications to this!
To name a few:
You need to catch the drips, else your bench gets even more messy..
Is that dripping fluid safe? (depends on your samples,
I would NOT recommend this in most clinical settings)
You CAN work with very small sample volumes...
Like most machinery, the standard instrument as delivered can often
be easily modified to do a particular job better - Provided you look
carefully at implications and side-effects.
Cheers, Joseph.
-- Joseph Webster Flow Cytometry Facility Centenary Institute
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web