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Vincent,
We sort chromosomes using Coulter EPICS V and 752 sorters, each
equipped with a 5-liter sheath tank. The large sheath capacity allows us to
sort for up to 33 hours at a time.
Our experience is that the position of the droplet becomes earlier
by about one half of a period during the course of a 33 hour sort. High
recovery and purity is maintained during these long sorts by deflecting two
drops and using the coincidence detection circuitry.
You might verify that this is the source of your instability by
varying the height of your sheath supply.
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John J. Fawcett, fawcett@lanl.gov National Flow Cytometry Resource
Life Sciences Division 505/667-9633 : office(vmail)
Los Alamos National Laboratory 505/667-2750 : LS Business Unit
P.O. Box 1663, ms M888 505/665-3024 : FAX
Los Alamos, NM 87545
USA
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web