FLUORESCENCE IMAGES OF MOUSE EMBRYOS
Robert M. Zucker and John Rogers
Reproductive Toxicology Division,
National Health and Environmental Effects Laboratory,
United States Environmental Protection Agency.
NOTE: This section contains some live 3D rotations that will operate under NETSCAPE or INTERNET Explorer browsers. Click on the Images to make them rotate. Rotations are fixed at 0.5 second per image-plane for these demonstrations.
Descriptions of the image files.
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A1A:3D image of 25 sections of a 9 day mouse embryo treated in vivo with MEOH and stained with lysotracker to show regions of cell death (phagocytosis activity of lysosomes). Magnification 100x and embryo thickness about 500 microns, pseudo color LUT. Note the cell death in the head region and the circular structures (otic pits) which will form the ears. |
| A1AB: Same as A1A but with a b/w. LUT.
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| A1C: Animated set of 3D rotations of data from A1A.
| Click on the picture to view 3-D rotation of image stack. If you don't see a rotating image, your browser doesn't support animated gifs |
| B1A: 3D image of sections of a 9 day mouse embryo that is over 700 microns thick. The embryo was stained with lysotracker to show regions of cell death (phagocytosis activity of lysosomes). Note the center line is region where the neural folds have joined and cells have died. Bright staining tissues are regions of cell death. Magnification 100x.
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| C1A: Embryo stained with acridine orange to similar to scanning electron microscope pictures magnification 25x. Note heart outside body and neural tube that is not open.
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| D1A: Control embryo showing apoptosis with lysotracker dye. magnification 100x.
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| E1A: Morphology of 9 day embryo stained with Nile blue. Note heart is out of the embryo body cavity. magnification 100x.
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| F1A: 8 day embryo treated with arsenic and stained with lysotracker to show regions of cell death. Magnification 100x.
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| G1A: Embryonic heart derived from embryo shown in E1A. Heart sections from a 9 day embryo stained with Nile blue and cleared with methyl salicylate. Magnification 200x.
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| G1C: Section of embryonic heart magnification 200x.
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| H1A: 11 day rat foetus demonstrating somites. Stained with acridine orange. magnification 25x.
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| L1A: 3D image of 25 sections of a 9 day mouse embryo treated in vivo with MEOH and stained with lysotracker to show regions of cell death (phagocytosis activity of lysosomes). Magnification 100x and embryo thickness about 500 microns, pseudo color LUT. Note the cell death in the head region and the circular structures (otic pits) which will form the ears. Shows individual sections that comprise the B1A 3D image. The sections are about 30 microns apart. Note that morphology and a specific apoptotic dye indicator can be measured.
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| L1C: Embryo treated with MeOH in vivo. An animated set of 3D images that have been made from L1A data set. The differences between the images are a few degrees in rotation.
| Click on the picture to view 3-D rotation of image stack. If you don't see a rotating image, your browser doesn't support animated gifs |
| M1A: 3D reconstruction of a 9 day mouse embryo's head that has been stained with Nile blue and cleared in methyl salicylate. Note that the individual sections comprising the head can be observed. This staining procedure is ideal for observing embryo morphology.
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Extra note.
| MB: See below:-
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| MBB: See below:-
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| M9MB: Individual sections from the M1A data set.
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Back to microscopy & image analysis.
CD-ROM Vol 3 was produced by Monica M. Shively and other
staff at the Purdue University Cytometry Laboratories and distributed free
of charge as an educational service to the cytometry community. If you
have any comments please direct them to Dr. J. Paul Robinson, Professor
& Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(765)
494-0757; FAX (765) 494-0517; Web
http://www.cyto.purdue.edu, EMAIL cdrom3@flowcyt.cyto.purdue.edu
