Purdue Cytometry CD-ROM Volume 3
Apoptosis Section advisor:
Dr. Zbigniew Darzynkiewicz

Analysis of apoptosis, or "programmed cell death", in which external factors can cause cells to become committed down a "suicide" pathway leading ultimately to cell death, increasingly has become a major application of flow cytometry. During this significant biological process, cellular DNA becomes fragmented; this low molecular weight DNA can leak out from the cells and be measured using flow cytometric techniques such as DNA labelling and enzyme-based assays. The purpose of this section is to provide a practical source of material relating to the flow cytometric analysis of this important phenomenon, which has implications for many areas of biology, including cell regulation and tumour proliferation.

  1. Methods in analysis of apoptosis and cell necrosis.  Z. Darzynkiewicz
  2. Common methods for measuring apoptotic cell death.  I. Nicoletti, et al.
  3. Combined measurement of apoptosis (phosphorylation) and cell cycle.   G. Basso
  4. In vivo measurements of tumour cell kinetics and apoptosis by flow cytometry.   N. Terry, et al.
  5. A rapid method for the measurement of apoptosis and necrosis by flow cytometry.   H. S. Kruger Gray, et al.
  6. Apoptosis in mouse lymphocytes.  M. Woda
  7. Detection of apoptosis in transgenic rats.  M.Woda
  8. Example data files showing analysis of apoptosis by flow cytometry.  H. S. Kruger Gray, et al.
  9. Selected email discussion of apoptosis

Note: These data have been provided by colleagues worldwide. Appropriate acknowledgement should be made when material is used.

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(765) 494-0757; FAX (765) 494-0517; Web http://www.cyto.purdue.edu, EMAIL cdrom3@flowcyt.cyto.purdue.edu