ISAC Specialty Training & Education Program

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FISH Methodology

Istituto di Genetica

Via Amendola 165/A

70126 B , ITALIA

Director: Dr. Mariano Rocchi

1. Facilities available: Molecular genetics laboratory; Sterile room for somatic cell hybrids cultures; Microscopes and digital imaging set-up (CCD camera).

2. Instrumentation: Axioplan microscope equipped with a cooled CCD camera; PowerMac computer and software for FISH image processing; In strumentation for molecular biology. Equipped sterile room for somitic cell hybrids cultures (used for producing sub chromosomal painting probes).

3. Specializition areas: Production of alphold centromeric probes specific for the centromeric regions of human chromosomes; Production of subchromosomal painting probes from somatic cell hybrids for FISH analysis of any cytogenetic rearrangement; Hundre ds of such probes are available at present.

4. Training Opportunities: FISH; Preparation of subchromosomal painting libraries from somatic cell hybrids; Handling of human-hamster somatic cell hybrids.

5. Special courses offered: Generation of somatic cell hybrids, Cytogenetic analysis of constitutional or acquired chromosome rearrangements through FISH analysis, with particular reference to subchromosomal painting probes.

6. Graduate Program: None.

7. Local Accomodations: Hotel Plaza, Piazza L. di Savoia, 15, Bari (85,000 Lit per night).

8. Transportation: Local airport is Bari Palese, with bus service to Bari.

9. Cost of Internships: Free.

10. Communication:

Dr. Mariano Rocchi

Istituto di Genetica

Universita di Bari

Via Amendola 165/A

70126 Bari, ITALIA

Phone: (39) 80 544 3371 FAX: (39) 80 544 3386 E-mail: rocchi@mvx36.csata.it

International Society for Analytical Cytology

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If you have suggestions or comments related to the STEP Program in general, problems with this particular page, or you want to know how to be a STEP program lab, send me an email message now robinson@flowcyt.cyto.purdue.edu

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(765) 494-0757; FAX (765) 494-0517; Web http://www.cyto.purdue.edu, EMAIL cdrom3@flowcyt.cyto.purdue.edu