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FIX & PERM®

Applications Guide to Intracellular Flow Cytometry

FIX & PERM® reagents are intended for fixing cells in suspension with Reagent A and then permeabilizing the cells with Reagent B. This procedure gives antibodies access to intercellular structures and leaves the morphological scatter characteristics of the cell intact. The unique formulation of FIX & PERM® reagents reduces background staining and allows simultaneous addition of permeabilization medium and fluorochrome labeled antibodies.

USE OF FIX & PERM® REAGENTS

STAINING PROTOCOLS

APPLICATIONS USING FIX & PERM®

TYPICAL FLOW CYTOMETRIC PATTERNS

SELECTED REFERENCES

Reagents Available From CALTAG

FIX & PERM® Permeabilization Kits

COMBI - IC® Monoclonal Antibodies:

For Intracellular Antigen Detection

For Intracellular Murine Cytokine Detection

For Intracellular Human Cytokine Detection

The FIX & PERM® reagent system and the COMBI-IC® antibodies were developed by AN-DER-GRUB Bioresearch, Austria. These products are offered worldwide as a partnership between CALTAG and AN-DER-GRUB.