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Course Description
BMS 631: Flow Cytometry - Theory.
A thorough excursion into the theory of flow cytometry. This course seeks to convey what the technology is, how it operates and where the applications exist. The course will cover optical systems, including filters, light excitation and emission, fluorescent dyes and fluorescent molecules, electronics including detection systems, signal processing, data analysis and computer applications. Lasers and other light sources as well as quality control will be discussed. Finally, an overview of the application areas in medical science, biological research, and other possible areas of use will be presented. There are NO PREREQUISITES.
BMS 632: Flow Cytometry - Practical Laboratory
A continuation course from the theory of flow cytometry, this course will involve a practical application of the technology. Students will be expected to be involved in a significant research project in which they learn the practical operation of a flow cytometer and produce their own data. It will be necessary for students to have a thorough understanding of the theory of flow cytometry before beginning this course. Aspects of quality control, adequate standards and references and an understanding of data analysis will be part of this course. PREREQUISITE: BMS 631.
At the end of the courses, all participants should be capable of understanding the theory and should be capable of operating instrumentation currently housed in the Cytometry Laboratories at Purdue University.
The courses are STRICTLY LIMITED because of the need for practical participation. Acceptance into the course will be at the instructor s discretion; however, current graduate students will be given priority.
BMS 631 - Lecture course : Jan 15-May 9, Tues, Thurs 3:30-5:00 (Dr. J. Paul Robinson unless otherwise indicated).
Week 1 Introduction
Week 2 History of Flow Cytometry
Week 3 Selection of parameters
Week 4 Optics
Week 5 Review and quiz
Week 6 Filters, PMTs, Light spectrum, Lasers
Week 7 Fluidic system, hydrodynamics, flow cells
Week 8 Precision, sensitivity, accuracy
Week 9 Review and quiz
Week 10-12 Multivariate Data Analysis
Week 13 Student seminars
Week 14 Review
Week 15 Final
BMS 632 - Lab/practical: Summer session, time to be arranged
(Lecture course is a prerequisite for lab course)
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