A) and B): metaphases of Feulgen-stained pea (Pisum sativum L.) root tip chromosomes observed by their fluorescent emission after green excitation, Standard and reconstructed karyotype L-84, respectively. C) and D): flow-karyotyping histograms of DAPI-stained chromosome suspensions for the Standard and L-84, respectively. Capital letters indicates chromosome specific peaks, as assigned after chromosome sorting.