Flow Cytometry and Microbiology
Plankton analysis by flow cytometry.
Michael E. Sieracki and Terry L. Cucci
J.J. MacIsaac Facility for Individual Particle Analysis
Bigelow Laboratory for Ocean Sciences
McKown Point, West Boothbay Harbor, ME 04575 USA
email: msieracki@bigelow.org
email: tcucci@bigelow.org
For more information about the J.J. MacIsaac Facility for Individual
Particle Analysis, please go to http://www.bigelow.org/~Sieracki
Data Examples
Below are examples of standard plots from flow cytometric analyses
of cultures and different plankton communities. All analyses were done
with a Becton-Dickinson FacScan flow cytometer. Two bivariate plots are
shown for each sample type: one of forward vs. side scatter, and the other
of forward scatter vs. chlorophyll fluorescence. Forward light scatter
is primarily related to cell size. Side scatter (measured at 90 degrees)
is a due to a variety of factors including refractive index, granularity
and cell shape. Chlorophyll fluorescence is present only in photosynthesizing
cells (i.e. plant or phytoplankton cells), or in cells that have ingested
phytoplankton.
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CD ROM Volume 4 was produced by staff at the Purdue University Cytometry
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