Step 2: Development Of A Rapid Test For Monitoring Wall Permeability

Wall permeability of Sphingomonas sp. 107 cells after fixation with paraformaldehyde. A: Wall permeability using ethidium brimide (EtBr). Fluorescence of non-permeabilized bacteria without EtBr (1), EtBr fluorecence in non-permeabilized (2) and permeabilized (3) cells. B: Fluorescent in situ hybridization (FISH) with the FITC-labeled specific SPHIN probe. Unpermeabilized (1) and permeabilized (2) Spingomonas sp. 107 cells.

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CD ROM Volume 4 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(765) 494-0757; FAX (765) 494-0517; Web http://www.cyto.purdue.edu EMAIL cdrominfo@flowcyt.cyto.purdue.edu