Cell type and dye help for ACAS?
sgaffin@NATICK-CCMAIL.ARMY.MIL
Tue, 14 Jan 97 09:53:16 EST
We are looking for cell types, preferably human, which adhere strongly
to glass coverslips at temperatures of 37oC and above and which are
resistant to leakage of fluorescent dyes. We found already that Na
Green, for instance, leaks at a very high rate from human macrophages,
lung epithelial cancer cells, and human cortical neurons. Similar
with SBFI and Indo-1 at anything above room temperature. We will be
grateful for any suggestions.
Steve Gaffin
US Army Research Institute of Environmental Medicine
Natick MA 01760-5007
Tel. 508-233-4867
fax 508-233-4869