I've been lurking around this E-mail list for about a month now enjoying
the discussions and the information being exchanged.
I have recently heard of a technique used in flow based in situ PCR
where the autofluorescence is greatly reduced by the addition of trypan
blue. It was just a casual reference in a talk and I was unable to find
anything more about it from the speaker.
I was wondering if there was anyone out there with experience in this
technique who could provide a protocol or a good reference for one.
While I have your ear, I would also appreciate any information that you
may have concerning epitope tagging reagents: anti-CDx-DIG/anti-DIG-PE
etc. If there are commercially available reagents I would be eager to
find out the sources.
Thank you for your time
Wallace Lauzon
Dept. Microbiology and Immunology
University of Ottawa
451 Smyth Rd
Ottawa, Canada