I have the company recommendation for small quantities of bleach to be
taken through the sample tubing (as if it was a sample). Then, I have
used 1% SDS 3 mls or so and then DDH20, where if the machine allows, I turn
it off with DDH20 in place (Calibur). For an Elite, I had a technician
who also applied Jet-Dry to the sample tubing and nozzle and I never once
doubted her in the claim of keeping salts from developing on the nozzle.
(She used, bleach, jet-dry, and DDH20 in that order prior to shut-down.)
As long a filters are by-passed, EtOH should be OK in pure analytical
machines. It is not recommended for any model of sorter. Despite any sort
of checklists, charged flow plates will often be left on and EtOH is the
*last* thing you want between charged plates as it a major fire hazzard.
As far a clogs go mid-run, I always keep that tube of 1-2% SDS handy.
David
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David L. Haviland, Ph.D.
Asst. Prof. Immunology
University of Texas - Houston, H.S.C.
Institute of Molecular Medicine
2121 W. Holcombe Blvd.
Houston, TX 77030
Internet:"dhavilan@imm2.imm.uth.tmc.edu"
Voice: 713.500.2413 FAX: 713.500.2424
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" Sometimes you're the windsheild, sometimes you're the bug."
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