Re: Fast Kinetic hardware

James Weaver 301-594-5879 FAX 301-594-3037 (WEAVER@cder.fda.gov)
Tue, 11 Mar 1997 14:06:20 -0400 (EDT)

I have actually used the Time Zero module in kinetic studies on an
Elite. It consists of a water jacketed cell holding the sample tube
with an induction type magnetic stirrer, control box, lines
connecting into the Elite air pressure lines, and BNC cables to
connect into the PMT signal lines. In my experience, a treatment can
be delivered to the sample tube, mixed, and delivered to the flow
cell in 2-3 seconds. I was able to perform a number of experiments
looking at cell volume regulation in a lymphoid cell line using
this component (see Weaver et al, Am J Physiol 270: (Cell Physiol
39): C1453-C1460, 1996).

If the events are happening in the 5-20 second time frame, this unit
will enable you to observe the cellular response to the treatment.
The unit was generally trouble free although I had to recut the air
lines occasionally at the T-connectors to remove slow air pressure
leaks. If you really need to work in this time frame, this unit will
work although it will take a little time to get it installed and get
used to it. I found the company (all 2 people) very helpful and
cooperative. Feel free to email if you need more information.

-Jim Weaver

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* James L. Weaver Ph.D. *
* Division of Applied Pharmacology Research *
* Office of Testing & Research *
* CDER MOD-1, FDA *
* 8301 Muirkirk Rd, Laurel MD 20708 *
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* Phone: 301-594-5879 *
* Fax: 301-594-3037 *
* Email:WEAVER@CDER.FDA.GOV *
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