conjugation vs. sandwich

Dave Novo (novod@muss.CIS.McMaster.CA)
Thu, 27 Mar 1997 00:06:24 -0500 (EST)

Hello everyone,

I have to buy an antibody for which it seems that there is no
company selling one pre-conjugated to a dye. I was wondering what people
thought of the relative merits of
conjugating FITC to the antibody myself vs.
buying a generic anti IgG-FITC premade conjugate and using a sandwich
type technique.

What are the pro's and cons of the various ideas?

Thanks in advance,
Dave

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