>
>I have some flow datas of healthy patients. These datas show, that
>HLA-DR negative cells have ~30 %. Other cells - HLA-DR positive are .
>
Most people would agree with Gary Woo that few if any T-cells in peripheral
blood of normal patients express HLA-DR. This is an activation antigen
which, in my experience, comes up relatively late (cells in
lectin-stimulated cultures may take 48 hours or more to express it). Ther
are always a few activated cells circulating in healthy people; perhaps 1%
of peripheral blood T-cells might express CD25, CD71, CD98, HLA-DR, and
other activation antigens. If you find 60-70% of cells from "healthy"
patients to be HLA-DR positive, either your "healthy" patients are in the
midst of a robust immune reaction or something went wrong with the analysis.
-Howard