>
> Hello,
> I need to measure the proliferation of a specific cell population
> (macrophages) and think that one of the bromo-deoxy-uridine and antibody
> kits would do the job.
>
> Does anyone have any recommendations about which manufacturer's kit does a
> good job? I have experienced variable quality in purchased kits, and
> would like to hear from someone who has actually used these before
> investing the money. Thanks!
>
> Rosemarie
>
Dear Rosemarie,
you don't need a kit for that. I use 10 mM BrdU from SIGMA in the
cell media and then the monoclonal BrdU antibody from Boehringer
Mannheim to do a PI-BrdU doublestaining. It works very nice and their
protocol is quite simple.
Ioakim Spyridopoulos, MD
Dept. of Cardiovascular Research
Boston, TUFTS University
email: ioakim@user1.channel1.com>