Re: HLA TYPING B27 BY FLOW

Andrew Lister (a.lister@worldnet.att.net)
Fri, 18 Apr 1997 22:16:15 -0400

ernest stapleton wrote:
>

> If any lab does screen for B27 there are other antibodies available
> (they also cross react with B7), namely Serotec and Becton Dickinson.
>St. John's NFLD

In addition to Serotec and BD, you may be interested to check
Coulter/Immunotech's IOTest HLA-B27/HLA-B7. This product is not
available in the USA, but I don't think that the restriction applies to
Canada.

> Our laboratory has also evaluated B27 typing by flow and have found
> there is no monospecific antibody to B27 yet. The one lambda clone
> also reacts with most hla B44 positives as well. Other labs have also
> found this cross reaction.

The combo reagent should help with the B7 cross reactivity
problem.

See //WWW.COULTER.COM/ss000212.htm#HLA-B27-HLA-B7/ if you are
interested.

Regards, Andrew Lister.