Re: Mouse splenocyte B/T cells

Steven Merlin (merlin@patho.unibe.ch)
Wed, 23 Apr 1997 13:06:22 +0200

On April 18th, Andrew Mitchell wrote:
>
>Can anyone give me an idea of the proportions of B and T cells (CD4/8 if
>you have(for strain: C57Bl/6I) should expect to find in normal mouse
>spleens. >I can't seem to find this data anywhere....
>
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Andrew,

Our experience with this mouse strain is 50-60% B cells and 40-50% T cells.
Of the T-cells, CD-4 is approx 66% and CD-8 is approx 33%.

Steven Merlin
University of Bern
FACS Core Facility
Murtenstraase 31
CH-3010 Bern, Switzerland
Lab: ++41-31-632-8876
Office: ++41-31-632-9960
FAX: ++41-31-381-8764
e-mail: merlin@patho.unibe.ch