RE: Proliferation index and apoptosis

Tom Mc Closkey (thomasm@nshs.edu)
Wed, 23 Apr 97 11:30:34 PDT

--- On Tue, 22 Apr 1997 08:54:03 -0600 Anna Travelstead
<travelst@wpsmtp.siumed.edu> wrote:
The PI wants
>DNA cell cycle analysis, proliferation index and apoptosis index
>performed. Does anyone have recommendations on the best apoptosis
>kit for use on a FACS Vantage?

The easiest way to measure these 3 parameters is to stain for DNA content
which allows you to quantitate apoptosis [below diploid] and proliferation
[above diploid] simultaneously. If you wish to analyze cell death using a
different method you have many choices, including TUNEL, annexin, which are
in kit form. There are also many other methods discussed in current
literature.

Good luck,
Tom

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Thomas W. Mc Closkey, Ph. D.
Director, Flow Cytometry
North Shore University Hospital
Biomedical Research Center
350 Community Drive
Manhasset, Long Island, New York 11030
ph: 516-562-4844 [office]; 516-562-1135/4641 [lab]
4/23/97 11:37:08 AM
E-mail: thomasm@nshs.edu
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