Re: Jurkat surface-marker

Kevin G Waddick (waddi002@maroon.tc.umn.edu)
Wed, 4 Jun 1997 10:36:45 -0500 (CDT)

Jurkat cells express CD2, which is a co-stimulatory adhesion receptor on
T-cells that has CD58 as its ligand. I am sure that you can get a FITC
conjugate of CD2 from virtually any source of MoAb; we got ours from
Coulter/Immunotech.

Kevin G. Waddick, Ph.D.
Staff Scientist
Hughes Institute
2685 Patton Road
Roseville, Minnesota 55113
(612) 628-0598
(612) 628-9891 Fax

On Tue, 3 Jun 1997 kjartan.egeberg@medisin.ntnu.no wrote:

>
> Dear flowers.
>
> In a flowcytometric study of adhetion of Jurkat cells to target cells, I
> need a good surface-marker that is direct conjugated to FITC. Jurkat cells
> do stain for CD3-FITC, but weak. They are negative for CD4, CD8, CD7 and
> CD45.
> A tip of a good marker will be highly appreciated !
>
> Thanks in advance and have a good flowday.
>
> Kjartan Egeberg
> Inst. of Canser Research and Mol. Biol.
> NTNU
> University in Trondheim
> E.mail: kjartan.egeberg@medisin.ntnu.no
>