RE: Jurkat marker

Calman Prussin (CPRUSSIN@atlas.niaid.nih.gov)
Thu, 5 Jun 1997 10:26:53 -0400

Do you need to use a surface marker? How about using one of the
membrane stable PKH dyes sold by Sigma? These are incredibly bright,
don't "bleed" from one cell to the next and are functionally inert.
You would just incubate your Jurkats in the dye prior to your adhesion
assay.

Calman

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From: kjartan.egeberg@medisin.ntnu.no
Sent: Wednesday, June 4, 1997 3:46 AM
To: cyto-inbox
Subject: Jurkat marker

Hi flowers.

Yesterday I sent a request for a good surface-marker to stain Jurkat
cells.
Rob McGilp wrote that CD95 is well expressed on these cells. Thank you
for
your reply. It is true that Jurkat express CD95, but since Fas/Fas-L
is
part of the study (adhetion of apoptotic cells), I have to use
another
marker to follow the Jurkats. I intend to use cell tracing. But I need
a
surface-marker to separate those adhered to targets from those that
are
been phagocytosed.

I'm still on my tip-toes with hopes for a good surface-marker.
Thanks.

Kjartan Egeberg
Inst. of Canser Research and Mol. Biol.
NTNU, Trondheim, Norway
E.mail: kjartan.egeberg@medisin.ntnu.no