On Mon, 9 Jun 1997, Kevin G Waddick wrote:
> Date: Mon, 9 Jun 1997 17:24:57 -0500 (CDT)
> From: Kevin G Waddick <waddi002@maroon.tc.umn.edu>
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> Cc: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> Subject: Re: double label for dendritic cells and MVE virus antigen
>
>
>
> If the infected cells co-express HLA-DR and CD83, they are dendritic cells.
>
> Kevin G. Waddick, Ph.D.
> Staff Scientist
> Hughes Institute
> 2685 Patton Road
> Roseville, Minnesota 55113
> (612) 628-0598
> (612) 628-9891 Fax
>
> On Mon, 9 Jun 1997, Leanne Sammels wrote:
>
> >
> > Hello,
> >
> > I'm not sure that this is the right place to post this question
> > but here goes. We have used in situ hybs to localise Murray
> > Valley encephalitis virus RNA in the lymph nodes of mice
> > infected IP with MVE. Only a small population of cells
> > within the lymph node are positive for viral RNA and the
> > morphology suggests it may be the follicular dendritic
> > cells. I am interested in using flow cytometry to analyse
> > infected lymph node cell suspensions(or maybe just
> > peritoneal washouts?)to see if it is the dendritic cells
> > which are replicating virus or if other cell populations
> > are also involved in viral replication. Is this feasible?
> > and if so can anyone recommend some publications which
> > would be helpful. I'm sure it is quite obvious by now
> > that I have had no cytometry experience whatsoever. Any
> > advice would be appreciated.
> > Kind regards,
> >
> > Lee Sammels
> > Dr Leanne Sammels
> > Department of Microbiology
> > University of Western Australia
> > Nedlands WA 6009
> >
> > Phone: 08 9346 4872
> > Fax: 08 9346 2912
> >
>