4-color flow

Darlene Steele-Norwood (steelen@fhs.csu.McMaster.CA)
Mon, 16 Jun 1997 11:57:56 -0400 (EDT)

I am having some problems in setting up the FacStar+ for 4-color flow
using FITC/PE/Tricolor/APC with argon and HeNe lasers and a 650LP
filter. We have been having considerable trouble detecting a Tricolor
signal, although a good signal can be detected using the same sample on
our FacScan. We have used the same antibody in biotinylated form with a
PE/Cychrome secondary (also on the FacStar+) with good results, but this
does not solve our problem as we need to use the biotin for the SA-APC
combination. Has anyone else experienced this problem using the
Tricolor? Any advice on instrument set-up or compensation would be
greatly appreciated.

Darlene Steele-Norwood
McMaster University
Department of Medicine
Hamilton, Ontario