> Using an orienting flow nozzle and staining for DNA content using
> Hoechst 33342 is the only reliable way to sort viable sexed sperm.
> While the idea of using oligos makes sense, the problem is that you
> have to kill the sperm to get the oligos into the DNA. No one to my
> knowledge has been able to come up with any specific surface markers
> that could be used to distinguish X from Y sperm without having to
> fix the sperm.
>
> Mike Fox
>
> On Mon, 16 Jun 97 11:56:27 -0600 CHELACK@admin3.usask.ca wrote:
>
> >
> > What kind of methods are being used to sort viable X and sperm in particular
> > bull sperm. A graduate student has brought me some older literature
> > describing sorting on differences in DNA content using specialized adaptors
> > to maintain consistent orientation of the sperm as they pass through the flow
> > cell. Is it possible to use FITC oligos to specific regions of the Y
> > chromosome to accomplish this? I have sent the graduate student back to search
> > out more on this possibility, but am wondering if anyone out there is already
> > doing this or has found it to be impossible, or is using another even more
> > elegant method? The graduate student is quite keen and quite green so I am
> > trying to make sure he isn't biting off more than he can chew.
> >
> >
> > regards
> >
> >
> > BJC - Brian
>
> Mike Fox
> Chairman, Cell and Molecular Biology Graduate Program
> Dept. of Radiological Health Sciences mfox@cvmbs.colostate.edu
> Colorado State University Ph: (970)491-7618
> Ft. Collins, CO 80523 USA Fax:(970)491-0623
> ====================================
>