809-753-9341
800-227-8143
FAX: (809) 758-3267
Abe Schwartz, Ph.D.
Flow Cytometry Standards Corporation
P.O. Box 194322
San Juan, PR 00919-4344
_______________________________________________________________________________
Subject: Comparisons of mean channel fluorescence
From: "Steven Shivers; Ph.D." <SHIVERS@aarlo.moffitt.usf.edu> at
Internet_Gateway
Date: 6/8/95 3:40 PM
A graduate student in our lab would like to compare the relative antigen
density of a marker on cells from a particular patient population
versus that of normal individuals. The patient and normal samples have
to be run on different days. I have explained to her the problems
inherent in this type of experiment: differences in instrument settings,
variabililty in staining conditions, the "relative" nature of
fluorescence intensity, etc. However, her mentor insists that this
type of experiment can and must be done.
Can anyone advise us on the best method for approaching this problem
by flow cytometry? Doesn't someone sell beads which are standardized
as to the amount of fluorescence on them? Can we use these as
internal standards to control for experiment-to-experiment
variability in fluorscence intensity of the antigen density of cells?
Steve Shivers
Bone Marrow Transplant Program
Moffitt Cancer Center
at the University of South Florida