1. We are doing PRA's by ELISA and the results compare to flow. In the past we
have occasionally done flow PRA's with a pool of 10+ fresh cells.
2. a. LR & cadaver = yes
b. CDC = yes
c. go with "FLOW"
d. Ficolled cells or flushed lymph node cells, both T & B cells.
Linda Buckert
Loma Linda Immunology Center
Loma Linda, CA 92354
email: Linda_Buckert@ccmail.llu.edu
______________________________ Reply Separator _________________________________
Subject: flow crossmatch
Author: Joanne.Luider@CRHA-Health.Ab.Ca at InternetMail
Date: 8/18/95 2:52 PM
We need advice/information on what's happening out in the world as far as
using flow cytometry crossmatch methods vs the cytotoxicity methods (CDC).
Some of our questions are:
1. Do you screen and/or identify PRA by flow?
-do you still use/report CDC results as well?
-what do you do when the flow result is positive and CDC is negative?
_do you use pooled random donor cells to screen? Fresh/frozen?
_advantages/disadvantages?
2. Do you do flow crossmatching prospectively for renal(or other organs)
tranplants for living related or cadaver donors?
_do you still use/report CDC results as well?
-what results are used if the results differ?
-what methodology is used for flow crossmatching? ficolled cells/whole
blood lysis? T and B?
Thanks in advance everyone!
Joanne Luider
Foothills Hospital
Calgary, Alberta
phone (403)670-4765
fax (403)270-4135