Does anyone have any experience using HBSS (Hanks Buffered Salt Solution)
as sheath in a sorter? We've been using it for some sorts over the last
couple of months because one of our users feels it increases cell viability.
We have problems phasing the side streams and it clogs our sheath filter.
Our usual buffer is PBS w/o Ca++ and Mg++ and we have no problems with it.
any comments would be appreciated
td
-- ============================================================================== Thomas Delohery | Internet: t-delohery@ski.mskcc.org Manager, Flow Cytometry Core Facility | Phone: (212) 639-8729 Memorial Sloan-Kettering Cancer Center | Fax: (212) 794-4019 1275 York Ave. Box 98 | New York, NY 10021 | ==============================================================================