fixated on fixation

Calman Prussin (CPRUSSIN@atlas.niaid.nih.gov)
Thu, 23 May 1996 17:32:03 -0400

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A question for the group:

Has anyone had experience with losing signal after fixing previously
stained cells? If so, with what fluorophore and what fixative? I have
been fixing cells with 2% methanol free paraformaldehyde and seem to be
losing my PE/Cy5 staining!

Thanks,

Calman

>calman@nih.gov

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