Request to Join CYTOMETRY from the web server
sstaylor@warren.med.harvard.edu
Sat, 20 Jul 1996 18:32:20 GMT
I would like to try a 3 colour experiment. The aim is to study the
effect of transfected constructs on cell cycle progression. So I need
to gate for transfected cells so I am cotransfecting a gfp construct
although I could us a CD2 construct. I need a DNA stain for cell cycle
analysis and I want to do an Ab labelling as well. Options I have come
up with so far are 1) gfp(transfected cells); PI (DNA) and Cy5(Ab); or
2)Cy3-CD2 (transfected cells) ; FITC (Ab) and Hoechst (DNA). Does
transfection of gfp, or mere transfection alone alter cell cycle
profiles? Any advice would be appreciated.