TNF receptors

Maurizio Mezzetti (mezz001@IT.net)
Wed, 7 Aug 1996 10:07:24 +0200

Hello everybody!
Has anybody had experience in detecting mouse membrane TNF receptors by flow
cytometry?
We have antibodies against the Type I and Type II human TNF receptors (TNFR)
and have found no reactivity with a mouse fibroblast cell line (A9 cells)
which is widely used for detecting TNF bioactivity, and therefore expresses
at least the Type I TNFR. It seems that we'll have to buy anti-mouse TNFR
MAbs, any particular suggestion regarding a specific clone, e.g. high
affinity binding? Any reference regarding expression of these receptors on
A9 cells would also help a lot!

Thanks for the help

Daniela Karrer
Istituto Di Ricerca C. Serono
Via di Valle Caia 22
00040 Ardea, Roma ITALY
tel +6-919491
fax +6-91949203
e-mail: mezz001@pn.itnet.it