Running a FACStar Plus with Dye laser at about 605nm;
I recently got a better pump laser, so was able to turn up the wick
on dye laser to about 150mW (I think!).
Wonderful improvement in both APC and Texas Red :-)
But now we are getting PKH-26 breaking into the APC channel! :-(
Strangely, not so much into the Texas Red channel... ;~}
I don't have a good picture of spectra for PKH-26, don't know if it is
absorbing at 605, but it does appear to have a long emission tail.
Any experience like this?
Ideas &/or info greatly appreciated.
Much Thanks, Joseph.
Joseph Webster (O.I.C. Flow Cytometry)
Centenary Institute of cancer Medicine & Cell Biology
Locked Bag No.6, Newtown, NSW 2042, AUSTRALIA.
Ph: 61-2-9565-6110 Fax: 61-2-9565-6101