PI fluorescence ratios
ssmurray@student.umass.edu
Mon, 07 Jul 1997 16:14:21 -0400
We are using Propidium Iodide to stain the DNA of fish and chicken blood
cells and then using the fish/chicken emission ratios to calculate the
amount of DNA in the fish cells. All this is being done on a
Becton-Dickinson FACScan with Cellfit software. We have noticed that the
FL2H (585 +/- 21 nm bandpass) and the FL3H (650 nm longpass) fish/chicken
ratios give us different answers. FL3H consistently gives us a lower ratio by
5-10%. Shouldn't they give us the same ratio?