hepatocytes
Christopher A. Worth (CAWORT01@ULKYVM.LOUISVILLE.EDU)
Thu, 15 Apr 93 12:01:22 EDT
Biomedical Engineer, BCC
Phone: (502)588-7191
reply to steve hilliard. You can punt the RBC's by lysing them or using gradie
nt separation. I'd try lysing first, since it is so much easier. Also if some
of the target cells get a bit lysed too, it shouldn't present much of aproblem
since you'd probably want to strip the cytoplasm from the nuclei in the first p
lace. If you'd like a good "recipe" for a lysing reagent let me know and I'll
dig it up for you.