good luck
Ugo Consoli
Istituto di Ematologia
Universita' di Catania, Italy
Fax: +39-95-7311510
Tel: +39-95-7435917
Dear collegues
>
>I wonder how many laboratories do the nuclear TdT stain
>by flow cytometric method after membrane permeabilization
>not by cytospin followed by fluorscence microscopic reading?
>
>In case of flow cytometric method, which protocol for membrane
>permeabilization you use?
>Is the permeabilization reagent Triton-X, saponin, FACS Lysing Solution,
>FACS Permeabilization Solution, Fix&Perm, or something special?
>
>And, have you ever tried to compare the results between
>flow cytometric method and cytospin method?
>
>Any comments will be appreciated.
>Thanks in advance.
>
>---------------------------------
>Chang-Seok Ki, M.D.
>Dept. of Clinical Pathology
>Samsung Medical Center
>Seoul, Korea
>Tel. 82-2-3410-2708
>Fax. 82-2-3410-2719
>E-mail. kcdol@samsung.co.kr
>---------------------------------