RE: Apoptosis... J'ai tombe aussi....

Bob Ashcroft (cytomat@netcore.com.au)
Thu, 18 Sep 1997 23:01:35 +1000

Lovely Howard, but what is that (C) for at the end? Are you aspiring to a second spring in Berlin?
Plus ca change, ...
On the grave of, ... ma sainte belle-mere!
Bob
-----Original Message-----
From: Howard Shapiro [SMTP:hms@shapirolab.com]
Sent: Wednesday, September 17, 1997 10:01 AM
To: Cytometry Mailing List
Subject: Apoptosis

Apoptosis takes its name from the Greek word describing the falling of
leaves or petals...which presented an irresistible opportunity for a Shapiro
flow song. I'm not sure many of you remember the Roger Williams piano
arrangement of "Autumn Leaves", but those of you who do can try to conjure
it up in the background.

-Howard

LES FEUILLES MORTS

When outer leaflets of cell membranes
Let phosphatidylserine show,
Labeled annexin V will bind there,
And you can measure it in flow.

Mitochondria deenergize
And superoxide levels rise,
But the nuclear signs of apoptosis
Come later; then, the cell dies.

Did thymocytes get radiation?
Was dexamethasone to blame?
Or was it simply fas ligation?
The end results are all the same.

Lytic enzymes in the cell are loosed,
And glutathione is reduced,
And, around the time the membrane's leaky,
The vultures all leave their roost.

The journals publish three new assays
For apoptosis every week;
Is it biology which varies
>From cell to cell, or just technique?

Cells can stay alive, as good as new,
If they can make bcl-2,
But, if not, their DNA's in fragments
When apoptosis is through.

(c) Howard Shapiro
September, 1997