CD34 antibody problems

Adrian Smith (A.Smith@centenary.usyd.edu.AU)
Mon, 22 Sep 1997 12:47:11 +1000

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>From: DEEKEWB@AOL.COM
>Date: Fri, 19 Sep 1997 00:51:49 -0400 (EDT)
>To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
>Subject: CD34 antibody problems
>
>
Is it possible that the CD34 epitope could
>have been blocked by HPCA-2 antibodies from the original sort? This
>explanation requires that PerCP dissociated from the antibody or
>photo-bleached. Is this possible?

We have had problems with PerCP photobleaching - if we leave our tubes in
the light while we are doing a run then the PerCP intensity of the last
sample will be dramatically lower than the first.

Adrian

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