cross-reactive MoAbs

Mara Rocchi (M.ROCCHI@CGNET.COM)
Thu, 25 Sep 1997 10:47:00 -0700 (PDT)

For Keith Bahjat, trough the cytometry list because my postmaster doesn't
like his address.
That's for the species cross-reactivity chart:

Dako Rabbit anti-human T cell, CD3, code n. A 452
cross react with bovine cells

This antibody recognize the intracytoplasmic portion of human CD3, and is
recommended for histopathology. I tested the antibody in flow cytometry on
bovine PBMC, by intracytoplasmic stain and it works very well. Double
staining (surface + cytoplasm) with anti-bovine CD3 showed positivity in
CD3 surface positive cells only. IgM positive cells (surface) were negative
for cytoplasmic CD3.

Hope this help. Cheers, and thanks for the work you're doing. Sometimes is
not easy for us (people that don't work with human, mouse o rat) to find the
antibody you're dreaming for.

Mara

Mara Rocchi, DVM Ph.D.,
Lab 4,
International Livestock Research Institute
P.O. box 30709
Nairobi, Kenya
Fax: 00254-2-631499
Mail: M.Rocchi@cgnet.com