I have been involved with flow cytometry since 1986 and during that whole
period have used, and have instructed the technicians I have taught to
use, 2.5% calf bovine serum in PBS for washing and final resuspension of
samples to be analyzed. Of course, we have gotten consistently good
results doing this, or we wouldn't have continued to do it. We normally
get tight population distributions, whether looked at as contour or dot
plots or as histograms, unless other factors such as instrumental or cell
sample quality problems are present. Our printouts are "publication
quality" and appear in many journal articles.
Kevin G. Waddick, Ph.D.
Staff Scientist
Hughes Institute
2685 Patton Road
Roseville, Minnesota 55113
(612) 628-0228 ext. 209
(612) 628-9891 Fax