RE: data display

Gib Otten (GibO@cellgenesys.com)
Tue, 30 Sep 1997 17:32:44 -0700

Somtheing that's been overlooked in this discussion. While a dot plot
by itself may be misleading because of the dot density problem, most
investigators identify regions of interest and indicate the percent
cells in those regions. The same should be done, and probably is being
done, with contour plots.

Gib Otten, Ph.D.
Senior Scientist, Gene Therapy Applications
Cell Genesys, Inc.
342 Lakeside Drive
Foster City, CA 94404
Tel: (415) 425-4515
email: gibo@mail.cellgenesys.com

> ----------
> From: Alice.L.Givan@dartmouth.edu
> Sent: Monday, September 29, 1997 9:48 AM
> To: Cytometry Mailing List
> Subject: re: data display
>
>
> I know that dot plots can be misleading for all the reasons that Mario
> Roederer
> describes --- BUT I also know that, by choice of the contouring
> algorithm, you
> can make a contour plot look any way you want: shoulders on peaks can
> be
> emphasized or can be made to disappear, double peaks can be made to
> look like
> single peaks, etc etc etc.. These problems are not solved by showing
> the dots
> that are below the contouring threshold, as they relate to the levels
> of
> coutours above the threshold. Dot plots can be misleading, but
> contour plots
> are a can of worms. OK Mario (and anyone else) -- looking forward to
> your
> response!
> Alice
>
> Alice L. Givan
> Englert Cell Analysis Laboratory
> Dartmouth Medical School
> Lebanon, New Hampshire
> NH 03756 USA
> tel 603-650-7661
> fax 603-650-6130
> e-mail givan@dartmouth.edu
>
>