Re[2]: large cell lymphoma
Maryalice Stetler-Stevenson (stetler@box-s.nih.gov)
Fri, 10 Oct 1997 10:00:40 -0400
We have not observed a big problem with large cell lymphoma yield-
i.e. we can flow them. This may be due to several unique advantages we
have. The lymph nodes are transported immediately from the O.R. to
Hematopathology where a fellow grosses it in and prepares a cell
suspension. We either process the cell suspension immediately or viably
freeze it. We may have some decrease in yield but we can detect the cells.
We get FNA's also and have no problem detecting large cell lymphoma. We
don't get great results with bone marrow with minimal involvement. If you
look for large cells as you are acquiring and just collect enough events to
have a sizeable large cell population you can detect it even with low
yields. This requires that you look at what you are putting through the
machine before running it. In our experience, the only real problem we have
had with large cell lymphomas is in minimal disease detection and we still
do well in this area at times.
Maryalice Stetler-Stevenson
Director Flow Cytometry Unit
Laboratory of Pathology, NCI, NIH